Pharmacia Biotech AKTA FPLC Purifier System P-920, UPC-900 & FRAC-900 Collector

Amersham Pharmacia Biotech AKTA FPLC Purifier System -P-920 (Code #: 18-1118-97) -UPC-900 (Code #: 18-1128-95) -FRAC-900 Collector (Code #: 18-1118-97) Made in Sweden Condition is used. Power on. No accessories. Pharmacia Biotech ÃKTA Purifier FPLC System Components: P-920 Pump, UPC-900 UV/pH/Conductivity Monitor, FRAC-900 Fraction Collector The ÃKTA Purifier FPLC system from Pharmacia Biotech is a modular, programmable liquid chromatography platform designed for the purification and analysis of biomolecules such as proteins, peptides, and nucleic acids. Built for flexibility, reproducibility, and precision, this system supports a wide range of chromatographic techniques including ion exchange, affinity, size exclusion, and hydrophobic interaction chromatography. ð§ Key System Components P-920 High-Performance Pump Dual-piston pump capable of delivering precise flow rates from 0.05 to 20 mL/min , ideal for analytical- to semi-preparative scale purification. Supports gradient elution with high accuracy and smooth flow, ensuring optimal resolution and reproducibility. Handles backpressures up to 50 bar , allowing compatibility with a variety of column formats and particle sizes. UPC-900 Monitor (UV, pH, and Conductivity Detection) Integrated optical detection system for UV absorbance at multiple selectable wavelengths (typically 214, 254, and 280 nm), useful for detecting proteins, peptides, and nucleic acids. Built-in conductivity and pH sensors provide real-time monitoring of buffer conditions, enabling precise gradient characterization. Allows simultaneous measurement of UV, pH, and conductivity for robust chromatographic profiling. FRAC-900 Automated Fraction Collector Programmable collector capable of fractionating based on volume, time, or peak detection criteria. Compatible with a wide variety of tube and rack sizes, offering flexibility for small-scale or bulk collection. Integrated with the system for synchronized collection, minimizing sample loss or misalignment during runs. 𧪠System Features and Benefits Modular Design : Components can be configured based on workflow needs; expandable for larger systems or more complex purification schemes. Software Control : Operated via UNICORN software for method creation, system control, data acquisition, and analysis. Versatile Applications : Purification of recombinant proteins, antibodies, enzymes, and nucleic acids. Buffer exchange and desalting. Analytical verification of sample purity and yield. Accurate Peak Monitoring : High-resolution chromatographic detection enables precise collection and characterization of target fractions. Reliable Performance : Robust design allows for long-term operation with minimal maintenance, suitable for research and routine lab environments. ð General Specifications (Typical Setup) Flow Rate Range : 0.05 to 20 mL/min Pressure Range : Up to 50 bar UV Detection : Dual-wavelength, 2 mm or 5 mm flow cell pathlength Conductivity Range : 1 µS/cm to 999.9 mS/cm Fraction Collection Modes : Time-based, volume-based, or peak-based Software : UNICORN compatible â Summary The Pharmacia Biotech ÃKTA Purifier system, equipped with the P-920 pump , UPC-900 monitor , and FRAC-900 fraction collector , offers a powerful, integrated solution for automated and high-precision liquid chromatography. With its modular architecture, versatile detection capabilities, and flexible fraction collection, it remains a benchmark system for FPLC-based biomolecule purification in both research and industrial laboratories.