BioTek Synergy H4 Hybrid Multi-Detection Microplate Reader

BioTek Synergy H4 Hybrid Multi-Detection Microplate Reader Sn: 238662 Made in USA Condition is used. Removed from a working lab. Untested - No accessories and power cord. BioTek Synergy H4 Hybrid Multi-Detection Microplate Reader The BioTek Synergy H4 Hybrid Multi-Detection Microplate Reader is an advanced, fully modular instrument engineered for superior sensitivity, flexibility, and throughput in multi-mode detection applications. It integrates both monochromator-based and filter-based optical systems in a single unit, allowing laboratories to optimize performance for a wide range of assay types â from routine absorbance measurements to complex fluorescence and luminescence studies. Hybrid Optical System The Synergy H4âs dual optical design combines the precision of wavelength selection via monochromators with the speed and sensitivity of optical filters. Users can easily switch between modes, making it ideal for labs that run diverse assays such as: Fluorescence intensity (FI) Time-resolved fluorescence (TRF) Fluorescence polarization (FP) Luminescence Absorbance (UV-Vis) This flexibility ensures maximum assay compatibility and future-proof versatility as research needs evolve. Performance and Sensitivity Equipped with a high-performance photomultiplier tube (PMT) and a dedicated xenon flash lamp , the Synergy H4 delivers exceptional signal-to-noise ratios and detection limits down to femtomolar concentrations. The filter-based path ensures high sensitivity for demanding assays, while the monochromator system offers precise wavelength control for spectral scanning and optimization. Assay and Plate Compatibility Supports 6- to 384-well microplates Variable pathlength correction for accurate quantitation Fast read speeds ideal for kinetic and endpoint assays Compatible with cell-based and biochemical assays Temperature control up to 65°C , along with orbital shaking , allows researchers to maintain consistent conditions for enzyme kinetics, cell viability, and reporter gene assays. Software and Data Management The reader is fully integrated with BioTekâs Gen5⢠Data Analysis Software , providing: Automated data collection and reduction Kinetic and endpoint analysis Spectral scanning and 3D visualization tools Easy export to Excel, LIMS, or other analysis platforms Gen5âs user-friendly interface simplifies complex protocol creation while maintaining GLP/GMP compliance and audit trails for regulated environments. Connectivity and Automation The Synergy H4 can be easily integrated into robotic and automated workflows through standard RS-232 and USB interfaces . Its compact footprint and modular design make it suitable for standalone use or as part of high-throughput screening systems. Technical Highlights Detection modes: FI, FP, TRF, Luminescence, Absorbance Excitation range: 250â850 nm (monochromator) Emission range: 300â850 nm (monochromator) Read speed: <20 seconds for 96-well fluorescence plate Dynamic range: >6 decades Temperature control: Ambient +4°C to 65°C Shaking: Linear, orbital, and double-orbital modes Compatible with Gen5 and third-party automation systems Applications ELISA and protein quantification Enzyme kinetics and inhibition studies Cell viability and cytotoxicity assays Reporter gene expression Fluorescence and luminescence-based assays DNA/RNA quantitation and molecular binding studies